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plasmid backbone dna  (New England Biolabs)


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    Structured Review

    New England Biolabs plasmid backbone dna
    Plasmid Backbone Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6042 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+backbone+dna/DpnI/pmc12802170-182-0-7
    Average 99 stars, based on 6042 article reviews
    plasmid backbone dna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Article Title: A family of linear plasmid phages that detect a quorum-sensing autoinducer exists in multiple bacterial species.
    Article Snippet: In cases in which inserts could not be generated by PCR, fragments were synthesized by Integrated DNA Technologies. .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces.
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL) at 37 °C for 30 minutes; 65 °C for 20 minutes. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10X CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hours and then 80 °C for 20 minutes, before gel extraction (0.8 % agarose gel, gel green dye, 80V, 100 minutes; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with the aforementioned phosphorylated duplex DNA pool (containing 200 fmol of each part variant and therefore sufficient to make 200 fmol of each design), nuclease-free water (40 μL), 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: A family of linear plasmid phages that detect a quorum-sensing autoinducer exists in multiple bacterial species
    Article Snippet: In cases in which inserts could not be generated by PCR, fragments were synthesized by Integrated DNA Technologies. .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Gel Extraction:

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL) at 37 °C for 30 minutes; 65 °C for 20 minutes. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10X CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hours and then 80 °C for 20 minutes, before gel extraction (0.8 % agarose gel, gel green dye, 80V, 100 minutes; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with the aforementioned phosphorylated duplex DNA pool (containing 200 fmol of each part variant and therefore sufficient to make 200 fmol of each design), nuclease-free water (40 μL), 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Agarose Gel Electrophoresis:

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL) at 37 °C for 30 minutes; 65 °C for 20 minutes. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10X CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hours and then 80 °C for 20 minutes, before gel extraction (0.8 % agarose gel, gel green dye, 80V, 100 minutes; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with the aforementioned phosphorylated duplex DNA pool (containing 200 fmol of each part variant and therefore sufficient to make 200 fmol of each design), nuclease-free water (40 μL), 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.

    Article Title: Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.
    Article Snippet: The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10× T4 DNA ligase buffer (2 μL) at 37 °C for 30 min; 65 °C for 20 min. .. Meanwhile, plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units; R3133S, New England Biolabs) in 10× CutSmart buffer (5 μL) and nuclease-free water (35 μL) at 37 °C for 4 hr and then 80 °C for 20 min, before gel extraction (0.8 % agarose gel, gel green dye, 80 V, 100 min; T1020S, New England Biolabs Monarch). .. Plasmid backbone (50 fmol) was used for pooled ligation based combinatorial assembly by combining with a 5-fold excess of the aforementioned phosphorylated duplex DNA pool (containing 250 fmol of each design for insertion into the plasmid), nuclease-free water (40 μL), 10× T4 DNA ligase buffer (5 μL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hr and then 65 °C for 10 min.

    Polymerase Chain Reaction:

    Article Title: A family of linear plasmid phages that detect a quorum-sensing autoinducer exists in multiple bacterial species.
    Article Snippet: In cases in which inserts could not be generated by PCR, fragments were synthesized by Integrated DNA Technologies. .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces.
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Article Snippet: In the case of pRE112, linear plasmid backbone was generated by restriction digestion with SmaI (NEB). .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..

    Article Title: A family of linear plasmid phages that detect a quorum-sensing autoinducer exists in multiple bacterial species
    Article Snippet: In cases in which inserts could not be generated by PCR, fragments were synthesized by Integrated DNA Technologies. .. Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA. ..



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